genetic analysis of aedes aegypti using random amplified polymorphic dna (rapd) markers from dengue outbreaks in pakistan

background: keeping in view the havoc situation of dengue fever in pakistan, the current study was designed to demon­strate the genetic variations, gene flow and rate of migration from lahore and faisalabad. methods: the larvae were collected from both natural and artificial breeding places from each collection site. the adult mosquitoes were collected by means of sweep net and battery-operated aspirator. dna extraction was per­formed using tne buffer method. ten genelink-a series rapd primers were used for pcr amplification and the data was analyzed through popgene. results: the number of amplification products produced per primer varied from 8-12, ranging from 200 to 2000 bp with an average of 10.0 bands per primer. the percentage of polymorphic loci amplified by each primer varied from 22.5 to 51%. the upgma dendrogram demonstrates two distinct groups from faisalabad and lahore populations. the genetic diversity ranged from 0.260 in faisalabad to 0.294 in lahore with a total heterozygosity of 0.379. the g st value for nine populations within lahore was 0.131 (nm= 3.317), whereas for nine populations in faisalabad g st value was 0.117 (nm= 3.773). the overall genetic variation among eighteen populations showed g st = 0.341 and nm= 1.966. conclusion: the genetic relatedness and nm value show that ae . aegypti populations exhibit intra-population gene flow both in faisalabad and lahore. although, both cities show a distinct pattern of genetic structure; however, few areas from both the cities show genetic similarity. the gene flow and the genetic relatedness in few populations of lahore and faisalabad cities need further investigation.